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Milosevic,Snezana; Subotic,Angelina; Bulajic,Aleksandra; Djekic,Ivana; Jevremovic,Sladjana; Vucurovic,Ana; Krstic,Branka. |
The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant... |
Tipo: Journal article |
Palavras-chave: Meristem culture; New Guinea Impatiens; Tomato spotted wilt virus; Virus- free plants. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003 |
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Sepúlveda R,Paulina; Larraín S,Patricia; Quiroz E,Carlos; Rebufel A,Patricia; Graña S,Fernando. |
Durante las temporadas de 2001-2002 y 2002-2003 se realizó una prospección de virus en pimientos (Capsicum annuum L.) en diversas localidades de la Región de Coquimbo (29,5-32° lat. Sur). La determinación de virus se realizó mediante la prueba DAS- ELISA. La importancia relativa de los virus encontrados para ambas temporadas fueron: Virus del mosaico del pepino (CMV) 23,3%; Virus del bronceado del tomate (TSWV) 20,8%; Virus del mosaico de la alfalfa (AMV) 14,8%;Virus Y de la papa (PVY) 14,5% e Impatients necrotic spot virus (INSV) 3,1% (primera identificación de este virus en pimiento en Chile); Virus del mosaico del tomate (ToMV) 2,2% y Virus del mosaico del tabaco (TMV) 4,9%. Los resultados indicaron que al menos el 30% de las muestras estaba infectada... |
Tipo: Journal article |
Palavras-chave: Virus en pimiento; Insectos vectores; Tomato spotted wilt virus; Cucumber mosaic virus; Alfalfa mosaic virus; Potato virus Y; ELISA. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072005000300001 |
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